3ETO
2 Angstrom Xray structure of the NOTCH1 Negative Regulatory Region (NRR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.927, 91.759, 59.844 |
| Unit cell angles | 90.00, 109.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.21313 |
| Rwork | 0.211 |
| R-free | 0.25185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oo4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 54862 | |
| <I/σ(I)> | 23.3 | 3.2 |
| Completeness [%] | 93.6 | 60.7 |
| Redundancy | 3.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1M Sodium acetate pH 4.0, 1.0-1.5M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
