Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2005-08-28 |
| Detector | NOIR-1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 65.642, 65.642, 101.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.130 - 1.700 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.22200 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.137 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.132 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.071 | 0.410 |
| Number of reflections | 25076 | |
| <I/σ(I)> | 7.6 | 1.6 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 6.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M MAGNESIUM CHLORIDE 0.1M TRIS- HCL 30% PEG 4000, PH 8.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K, pH 8.50 |
