3ESW
Complex of yeast PNGase with GlcNAc2-IAc.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 131.530, 131.530, 127.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.960 - 3.400 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23500 |
| Starting model (for MR) | 1x3z |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.599 |
| Data reduction software | DENZO |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.969 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.126 | 0.457 |
| Total number of observations | 20859 | |
| Number of reflections | 17986 | |
| <I/σ(I)> | 5.496 | 4.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 291 | 0.1 M Tris-HCl, pH 8.5 and 2.0 M sodium chloride, EVAPORATION, temperature 291K |
