3ES8
Crystal structure of divergent enolase from Oceanobacillus Iheyensis complexed with Mg and L-malate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 105.584, 105.695, 105.718 |
| Unit cell angles | 109.31, 109.47, 109.66 |
Refinement procedure
| Resolution | 24.940 - 2.200 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.079 |
| Number of reflections | 170773 |
| Completeness [%] | 95.7 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 0.15 M DL-malic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
