3ES6
Crystal structure of the novel complex formed between Zinc 2-glycoprotein (ZAG) and Prolactin inducible protein (PIP) from human seminal plasma
Replaces: 2ICNExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 132.298, 132.298, 73.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.230 |
| R-factor | 0.20033 |
| Rwork | 0.196 |
| R-free | 0.24041 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zag |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.302 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.250 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 10296 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 50mM sodium acetate, 20% PEG4000, pH6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
