3ES2

Structure of the C-terminal phosphatase domain of P. aeruginonsa RssB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	.97930
Spacegroup name	P 31
Unit cell lengths	70.211, 70.211, 92.924
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.950 - 2.950
R-factor	0.259
Rwork	0.257
R-free	0.29600
RMSD bond length	0.004
RMSD bond angle	0.756
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	13.950
High resolution limit [Å]	2.950
Number of reflections	19831
Completeness [%]	92.6
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.0 M Lithium Sulfate, 0.1 M MES, cryo-protected with 25% glycerol in well solution, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.0 M Lithium Sulfate, 0.1 M MES, cryo-protected with 25% glycerol in well solution, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K