3EQT

Crystal structure of human LGP2 C-terminal domain in complex with dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2008-05-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	C 1 2 1
Unit cell lengths	116.458, 54.191, 67.203
Unit cell angles	90.00, 97.26, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.214
Rwork	0.213
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2QFB
RMSD bond length	1.430
RMSD bond angle	0.008
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.046	0.336
Number of reflections	28126
<I/σ(I)>	0.462	3.5
Completeness [%]	99.6	98.6
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.2 M ammonium sulfate, 16-18% PEG3350, 0.1 M Tris-HCl buffer, protein concentration 30 mg/ml, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	0.2 M ammonium sulfate
2	1	1	PEG3350
3	1	2	0.2 M ammonium sulfate
4	1	2	PEG3350