3EQS
Crystal structure of human MDM2 in complex with a 12-mer peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.517, 43.274, 35.036 |
| Unit cell angles | 90.00, 116.51, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| R-factor | 0.1561 |
| Rwork | 0.154 |
| R-free | 0.19276 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ycr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.524 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.850 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.058 | 0.217 |
| Number of reflections | 11993 | |
| <I/σ(I)> | 46.9 | 13.6 |
| Completeness [%] | 99.5 | 95.1 |
| Redundancy | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2 M Ammonium acetate, 0.1M Sodium citrate, 30% PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
