3EPZ
Structure of the replication foci-targeting sequence of human DNA cytosine methyltransferase DNMT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-27 |
| Detector | MARMOSAIC 300 MM CCD QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.544, 59.768, 96.286 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 48.110 - 2.310 |
| R-factor | 0.21559 |
| Rwork | 0.213 |
| R-free | 0.26413 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.154 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 28832 | |
| <I/σ(I)> | 16.6849 | 1.3 |
| Completeness [%] | 99.1 | 91.6 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | 23 % PEG 3350, 0.1 M BIS-TRIS PH 6.0,0.2 M SODIUM ACETATE, 0.005 M TCEP, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K, pH 6.00 |
