3EPG
Structure of Human DNA Polymerase Iota complexed with N2-ethylguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.535, 98.535, 202.354 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.23671 |
| Rwork | 0.234 |
| R-free | 0.28284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ALZ minus DNA |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.806 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.122 | 0.400 |
| Number of reflections | 19625 | |
| <I/σ(I)> | 13 | 5.1 |
| Completeness [%] | 98.9 | 90.6 |
| Redundancy | 17.77 | 11.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 12-15% PEG MME 5000, 0.2-0.4M Ammonium Sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG MME 5000 | ||
| 2 | 1 | 1 | Ammonium Sulfate | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 2 | PEG MME 5000 | ||
| 5 | 1 | 2 | Ammonium Sulfate | ||
| 6 | 1 | 2 | MES |
