3EP1
Structure of the PGRP-Hd from Alvinella pompejana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.360, 71.850, 141.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.960 - 2.100 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yck |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.260 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 21905 | |
| <I/σ(I)> | 15.7 | 5.7 |
| Completeness [%] | 98.5 | 96.7 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 200mM Sodium Tartrate, 20% PEG3350, pH7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
