3EOY
Structure of Reovirus sigma1 in Complex with Its Receptor Junctional Adhesion Molecule-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-26 |
| Detector | MAR225 CCD |
| Wavelength(s) | 0.91988 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.940, 124.340, 130.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2OJ5 and 1NBQ |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.543 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.163 | 0.212 |
| Number of reflections | 21974 | |
| <I/σ(I)> | 6.933 | 2.7 |
| Completeness [%] | 90.2 | 69.7 |
| Redundancy | 3.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1 M CHES, 30% PEG 3350, streak seeding, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
