3EOJ

Fmo protein from Prosthecochloris Aestuarii 2K AT 1.3A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 63
Unit cell lengths	111.242, 111.242, 98.198
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.300
R-factor	0.1365
Rwork	0.135
R-free	0.16130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1BCL
RMSD bond length	0.013
RMSD bond angle	0.030
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.074	0.596
Number of reflections	168292
<I/σ(I)>	26.5	2.77
Completeness [%]	99.9	100
Redundancy	7.8	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	Well Solution: 10mM Tris/HCL pH 8.0, 1.0M NaCl, 10% w/v (NH4)2SO4. Protein Solution: 10mM Tris/HCL pH 7.8 1.0M NaCl at 14mg/ml of complex. Drop composed of 5ul of protein solution and 3ul of well solution., VAPOR DIFFUSION, HANGING DROP, temperature 298K