3EOH

Refined group II intron structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-17
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	89.092, 94.969, 225.972
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.705 - 3.125
R-factor	0.2095
Rwork	0.208
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.397
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.705
High resolution limit [Å]	3.125
Number of reflections	34739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	303	6% PEG 3350, 150 mM magnesium acetate, 350 mM KCL, 50 mM Na-HEPES pH 7.0, 0.5 mM spermine, VAPOR DIFFUSION, SITTING DROP, temperature 303K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 3350
2	1	1	magnesium acetate
3	1	1	KCL
4	1	1	Na-HEPES
5	1	1	spermine
6	1	2	PEG 335
7	1	2	magnesium acetate
8	1	2	KCL