3EOE

Crystal Structure of Pyruvate Kinase from toxoplasma gondii, 55.m00007

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	98.039, 130.684, 113.848
Unit cell angles	90.00, 117.40, 90.00

Refinement procedure

Resolution	42.680 - 2.310
R-factor	0.208
Rwork	0.206
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.118
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.310	2.310
Rmerge	0.069	0.588
Number of reflections	103408
<I/σ(I)>		1.2
Completeness [%]	93.2	53.8
Redundancy	3.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1