3EOD
Crystal structure of N-terminal domain of E. coli RssB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932,1.0070,1.0087 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.114, 45.114, 96.797 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.070 - 1.750 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.839 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.061 | 0.044 | 0.464 |
| Number of reflections | 12003 | ||
| <I/σ(I)> | 80.733 | ||
| Completeness [%] | 99.1 | 94.8 | 98 |
| Redundancy | 38.6 | 31.3 | 23.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.75 | 298 | 0.1 M Tris-HCl, 0.2 Na Acetate, 1 mM DTT, 28% PEG3350, 1.9 mg/ml protein, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
