3EOC
Cdk2/CyclinA complexed with a imidazo triazin-2-amine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 182.977, 182.977, 213.687 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.21585 |
| Rwork | 0.215 |
| R-free | 0.23836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.110 | 0.610 |
| Number of reflections | 33305 | |
| <I/σ(I)> | 14.9 | 2.1 |
| Completeness [%] | 98.9 | 99 |
| Redundancy | 4.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 1.5 M Sodium acetate, 100 mM Sodium cacodylate pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
