3ENB

Crystal Structure of PRP8 core domain IV

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.115872, 0.9796, 1.0200, 0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	75.397, 78.062, 93.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.980 - 1.850
R-factor	0.208
Rwork	0.206
R-free	0.24300
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.224
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.920
High resolution limit [Å]	1.850	3.990	1.850
Rmerge	0.044	0.021	0.408
Number of reflections	47833
<I/σ(I)>	27.879
Completeness [%]	99.7	98.8	99.6
Redundancy	3.9	3.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	2.5 M NaCl, 100 mM Tris-HCl pH 7.0, 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 296K