3EN3
Crystal Structure of the GluR4 Ligand-Binding domain in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.906, 48.749, 47.766 |
| Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
| Resolution | 11.950 - 2.430 |
| R-factor | 0.159 |
| Rwork | 0.156 |
| R-free | 0.21900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.950 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 10025 | |
| Completeness [%] | 95.3 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 25.5% PEG 1500, 0.05M Na-Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
