3EMN

The Crystal Structure of Mouse VDAC1 at 2.3 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	298
Detector technology	CCD
Collection date	2008-06-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	100.230, 58.390, 66.580
Unit cell angles	90.00, 99.25, 90.00

Refinement procedure

Resolution	19.510 - 2.300
R-factor	0.244
Rwork	0.242
R-free	0.27700
Structure solution method	SIRAS
RMSD bond length	0.014
RMSD bond angle	1.768
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.510	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.029	0.471
Number of reflections	16629	2010
<I/σ(I)>		2.6
Completeness [%]	97.5	98.7
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	18% MPD, 0.1 M Tris-HCl, 10% PEG400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	18% MPD, 0.1 M Tris-HCl, 10% PEG400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	18% MPD, 0.1 M Tris-HCl, 10% PEG400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K