3EL8
Crystal structure of c-Src in complex with pyrazolopyrimidine 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.375, 63.693, 73.792 |
| Unit cell angles | 101.01, 90.25, 90.12 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.2183 |
| Rwork | 0.218 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yoj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 31919 |
| <I/σ(I)> | 12.5 |
| Completeness [%] | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 287 | 100 mM NaCl, 10 mM Tris-HCl, 5% Glycerol, 2 mM DTT, 4% DMSO, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
