3EKU

Crystal Structure of Monomeric Actin bound to Cytochalasin D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-09-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	207.400, 54.040, 36.890
Unit cell angles	90.00, 98.96, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.201
Rwork	0.177
R-free	0.22800
Starting model (for MR)	2hf4
RMSD bond length	0.006
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.108	0.278
Number of reflections	13808
<I/σ(I)>	16.1	3.2
Completeness [%]	97.9	85.8
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	277	20% MPD, 0.1M sodium acetate, 0.1M NaCl, 0.02M CaCl2, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	5.2	277	20% MPD, 0.1M sodium acetate, 0.1M NaCl, 0.02M CaCl2, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K