3EKK
Insulin receptor kinase complexed with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-03-21 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.251, 70.487, 88.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.130 - 2.100 |
| R-factor | 0.20723 |
| Rwork | 0.204 |
| R-free | 0.26021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p14 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.105 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.130 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.098 | 0.460 |
| Number of reflections | 20779 | |
| <I/σ(I)> | 28 | 6.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1 M MOPS, 1.0 M Na3citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
