3EKJ
Calcium-free GCaMP2 (calcium binding deficient mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.871, 47.674, 42.991 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 31.880 - 2.800 |
| R-factor | 0.21272 |
| Rwork | 0.209 |
| R-free | 0.28043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.678 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.940 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 10580 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 98.9 | 98.6 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
