3EK8
Calcium-saturated GCaMP2 T116V/G87R mutant monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-12 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.800, 120.800, 97.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.800 |
| R-factor | 0.22569 |
| Rwork | 0.224 |
| R-free | 0.26553 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.355 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 18310 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.94 | 12.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1 M Magnesium formate dihydrate, 15% w/v Polyethylene glycol 3,350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
