3EK2
Crystal structure of eonyl-(acyl carrier protein) reductase from burkholderia pseudomallei 1719b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-01 |
| Detector | MAR 300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.195, 122.195, 139.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.673 - 1.900 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21800 |
| Structure solution method | MR, MR |
| Starting model (for MR) | 1qsg tetramer modified |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.507 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.673 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.900 |
| Rmerge | 0.142 | 0.598 |
| Number of reflections | 82541 | |
| <I/σ(I)> | 3.7 | 1 |
| Completeness [%] | 95.5 | 97.7 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | JCSG+ SCREEN: 10% PEG 6000, 100MM HEPES PH 7.0, VAPOR DIFFUSION, TEMPERATURE 298K, pH 7.00, VAPOR DIFFUSION, SITTING DROP |
