3EIP
CRYSTAL STRUCTURE OF COLICIN E3 IMMUNITY PROTEIN: AN INHIBITOR TO A RIBOSOME-INACTIVATING RNASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-08 |
| Detector | SDMS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.070, 53.720, 36.250 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 44.600 - 1.800 |
| Rwork | 0.218 |
| R-free | 0.26500 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.400 * |
| Data reduction software | SDMS |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.4A) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.167 * |
| Number of reflections | 11860 * | |
| <I/σ(I)> | 41.1 | 2.5 |
| Completeness [%] | 81.0 * | 77 * |
| Redundancy | 2.03 | 1.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 2.0 M AMMONIUM SULFATE, 0.1 M MES BUFFER, PH 6.0, pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | sodium azide | 10 (mM) | |
| 2 | 1 | drop | ammonium sulfate | 1.4 (M) | |
| 3 | 1 | drop | sodium chloride | 80 (mM) | |
| 4 | 1 | drop | MES | 33 (mM) | |
| 5 | 1 | drop | sodium azide | 0.005 (mM) | |
| 6 | 1 | drop | PMSF | 0.005 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 8 | 1 | reservoir | MES | 100 (mM) | |
| 9 | 1 | reservoir | sodium chloride | 100 (mM) |
