3EIN
Delta class GST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.775, 63.212, 54.513 |
| Unit cell angles | 90.00, 129.84, 90.00 |
Refinement procedure
| Resolution | 31.610 - 1.126 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.170 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.048 | 0.196 |
| Number of reflections | 69523 | |
| Completeness [%] | 99.2 | 94 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 25%(w/v) PEG3350, 0.2M NaCl, 100mM HEPES, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
