3EI8

Crystal structure of K270N variant of LL-diaminopimelate aminotransferase from Arabidopsis thaliana complexed with LL-DAP: External aldimine form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-30
Detector	ADSC QUANTUM 315
Wavelength(s)	1.115872
Spacegroup name	P 32 2 1
Unit cell lengths	102.476, 102.476, 171.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.410 - 1.600
R-factor	0.16819
Rwork	0.167
R-free	0.18769
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z20
RMSD bond length	0.009
RMSD bond angle	1.222
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0055)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	137740
Completeness [%]	99.8	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	45% (NH4)2SO4, 0.1 M HEPES pH 7.5, 3% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K