3EI1
Structure of hsDDB1-drDDB2 bound to a 14 bp 6-4 photoproduct containing DNA-duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 112.740, 123.600, 158.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.27800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.590 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 56150 | 5526 |
| <I/σ(I)> | 23.4 | 4.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 100mM Ca-Acetate, 100mM MES pH 5.7, 12-14 % PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ca-Acetate | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 2 | Ca-Acetate | ||
| 5 | 1 | 2 | MES | ||
| 6 | 1 | 2 | PEG 400 |
