3EHZ

X-ray structure of the pentameric ligand gated ion channel of Gloebacter violaceus (GLIC) in a presumptive open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-05-26
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	179.225, 132.864, 161.422
Unit cell angles	90.00, 102.19, 90.00

Refinement procedure

Resolution	19.972 - 3.100
R-factor	0.239
Rwork	0.238
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vl0
RMSD bond length	0.010
RMSD bond angle	1.378
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	48.850	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge	0.092	0.037	0.607
Total number of observations		12519	62851
Number of reflections	66826
<I/σ(I)>	5.28	10.1	1.1
Completeness [%]	99.6	98	99.9
Redundancy	6.3	5.8	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	277.15	9% PEG4000, 50mM CH3COONa, 200mM (NH4)2SO4, pH4.0, vapor diffusion, hanging drop, temperature 277.15K, VAPOR DIFFUSION, HANGING DROP