3EHE
Crystal structure of UDP-glucose 4 epimerase (galE-1) from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.574, 55.529, 89.634 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 34.460 - 1.870 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.060 | 0.381 |
| Number of reflections | 54749 | |
| <I/σ(I)> | 12.1 | 1 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 7.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 25% Ethylene Glycol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
