3EH2
Crystal structure of the human COPII-coat protein Sec24c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2007-05-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.690, 182.980, 201.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.780 - 2.350 |
Rwork | 0.241 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.047 | 0.346 |
Number of reflections | 104257 | |
<I/σ(I)> | 27.8 | 3.6 |
Completeness [%] | 99.4 | 98.3 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | CRYSTALS WERE GROWN VIA THE HANGING-DROP METHOD, BY ADDING 1UL PROTEIN SOLUTION TO 1UL OF WELL SOLUTION COMPRISING 6% (W/V) PEG 4000 AND 0.1 M MES., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |