3EGN
C-terminal RNA Recognition Motif of the U11/U12 65K Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.973, 33.499, 49.312 |
| Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.19879 |
| Rwork | 0.194 |
| R-free | 0.23619 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1urn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.108 | 0.563 |
| Number of reflections | 5299 | |
| <I/σ(I)> | 9.6 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 20% (w/v) PEG 3350, 0.2M LiCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
