3EFM
Structure of the alcaligin outer membrane recepteur FauA from Bordetella pertussis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | FLAT PANEL |
| Collection date | 2008-05-16 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 165.893, 188.847, 62.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.350 - 2.330 |
| R-factor | 0.22091 |
| Rwork | 0.219 |
| R-free | 0.25843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5p |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.565 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.420 | 2.400 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 42320 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.75% C8E4, 0.1M HEPES, pH7.5, 0.3-0.5M NH4SO4, 13-17% MPD, protein concentration 10mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
