3EFG
Structure of SlyX protein from Xanthomonas campestris pv. campestris str. ATCC 33913
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97931, 0.91966, 0.91948 |
Spacegroup name | P 63 |
Unit cell lengths | 35.945, 35.945, 81.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.130 - 2.000 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.21100 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.389 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.051 | 0.533 |
Number of reflections | 4751 | |
<I/σ(I)> | 58.469 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 12.4 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris pH 8.5, 0.2M Ammonium Sulfate, 20% PEG 3350, 4% Glycerol, 1/60 cymotrypsin, then soaked in Na Bromide for phasing, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris pH 8.5, 0.2M Ammonium Sulfate, 20% PEG 3350, 4% Glycerol, 1/60 cymotrypsin, then soaked in Na Bromide for phasing, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris pH 8.5, 0.2M Ammonium Sulfate, 20% PEG 3350, 4% Glycerol, 1/60 cymotrypsin, then soaked in Na Bromide for phasing, VAPOR DIFFUSION, HANGING DROP, temperature 291K |