3EES
Structure of the RNA pyrophosphohydrolase BdRppH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-09 |
| Detector | adsc Q210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.491, 70.491, 100.481 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.260 - 1.900 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.25600 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.298 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.260 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.043 | 0.033 | 0.390 |
| Number of reflections | 23316 | ||
| <I/σ(I)> | 63.364 | ||
| Completeness [%] | 99.8 | 98 | 100 |
| Redundancy | 10 | 10 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4 | 298 | PEG 4000, Na acetate, hanging drop, temperature 298K |
