3EEE
Probing the function of heme distortion in the H-NOX family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.253, 124.686, 83.555 |
Unit cell angles | 90.00, 126.58, 90.00 |
Refinement procedure
Resolution | 45.672 - 2.120 |
R-factor | 0.2071 |
Rwork | 0.205 |
R-free | 0.25190 |
Starting model (for MR) | 1u55 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.898 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.055 | 0.455 |
Number of reflections | 53717 | |
<I/σ(I)> | 2.7 | |
Redundancy | 4.3 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9.1 | 289 | 0.1 M NaSCN, 0.1 M Tris pH 9.1, 0.2 M (NH4)2(SO4), and 18% (w/v) PEG 8000 at 289K |