3EDU

Crystal structure of the ankyrin-binding domain of human erythroid spectrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	77
Detector technology	CCD
Collection date	2008-06-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	F 2 2 2
Unit cell lengths	74.823, 111.746, 127.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.000 - 2.100
R-factor	0.2148
Rwork	0.210
R-free	0.25180
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	15696
<I/σ(I)>		3
Completeness [%]	99.2	96.3
Redundancy	11.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	289	0.1M bis-tris-propane, pH 6.2, 0.2M KSCN, 20% PEG 3,350, 3-10mM spermine, VAPOR DIFFUSION, SITTING DROP, temperature 289K
1	VAPOR DIFFUSION, SITTING DROP	6.2	289	0.1M bis-tris-propane, pH 6.2, 0.2M KSCN, 20% PEG 3,350, 3-10mM spermine, VAPOR DIFFUSION, SITTING DROP, temperature 289K