3EDI
Crystal structure of tolloid-like protease 1 (TLL-1) protease domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.281, 58.923, 47.444 |
| Unit cell angles | 90.00, 104.62, 90.00 |
Refinement procedure
| Resolution | 29.960 - 1.400 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dle |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.473 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.900 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.073 | 0.479 |
| Number of reflections | 41793 | |
| <I/σ(I)> | 15.9 | 2.9 |
| Completeness [%] | 96.3 | 74.1 |
| Redundancy | 3.5 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 25% PEG 3350, 200mM lithium sulphate, 100mM Bis-Tris-HCl, pH 7.5, vapor diffusion, temperature 277K |
