3ED5
The crystal structure of YfnB from Bacillus subtilis subsp. subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-08 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 65 |
| Unit cell lengths | 91.186, 91.186, 68.253 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.820 - 1.720 |
| R-factor | 0.17245 |
| Rwork | 0.171 |
| R-free | 0.19725 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.740 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.089 | 0.740 |
| Number of reflections | 34185 | |
| <I/σ(I)> | 32.2 | 1.82 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 3.5M Sodium Format 0.1M Sodium Acetate Trihydrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
