3ECP
Crystal Structure Of Tn5 Transposase Complexed With 5' Phosphorylated Transposon End DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00964 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 113.508, 113.508, 228.775 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.22153 |
| Rwork | 0.219 |
| R-free | 0.27386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1muh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.529 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.044 | 0.379 |
| Number of reflections | 30992 | |
| <I/σ(I)> | 69.5 | 9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.4 | 18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 13% (w/v) oMe-PEG 5000, 0.1M bis-tris, 0.1M ammonium sulfate, pH 6.5, microbatch, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | oMe-PEG 5000 | ||
| 2 | 1 | 1 | bis-tris | ||
| 3 | 1 | 1 | (NH4)2SO4 | ||
| 4 | 1 | 2 | oMe-PEG 5000 | ||
| 5 | 1 | 2 | bis-tris | ||
| 6 | 1 | 2 | (NH4)2SO4 |
