3EC2
Crystal structure of the DnaC helicase loader
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795, 1.11587 |
Spacegroup name | P 61 |
Unit cell lengths | 84.492, 84.492, 51.113 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
Rwork | 0.233 |
R-free | 0.27800 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.985 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.250 |
Number of reflections | 5534 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 50mM Tris, 1% PEG 6K, 1mM TCEP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |