3EBT

Crystal structure of a ntf2-like protein of unknown function (bpss0132) from burkholderia pseudomallei k96243 at 1.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97848,0.97904
Spacegroup name	P 31 2 1
Unit cell lengths	75.730, 75.730, 50.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.431 - 1.300
R-factor	0.141
Rwork	0.140
R-free	0.16200
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.760
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.431	27.431	1.350
High resolution limit [Å]	1.300	2.800	1.300
Rmerge	0.062	0.049	0.481
Rmeas	0.078	0.061	0.607
Total number of observations	219462
Number of reflections	40949	7739	8321
<I/σ(I)>	9.59	20.6	2.1
Completeness [%]	99.0	97.8	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20.0000% Glycerol, 0.0400M KH2PO4, 16.0000% PEG-8000, No Buffer pH None, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K