3EB8

VirA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	91.290, 91.290, 198.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.990 - 2.400
R-factor	0.22
Rwork	0.217
R-free	0.26700
Structure solution method	SIRAS
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.054	0.386
Number of reflections	33386
<I/σ(I)>	27	2.07
Completeness [%]	99.5	98
Redundancy	6.3	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1