3EB5

Structure of the cIAP2 RING domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-09-22
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.2827,1.1808,1.2829
Spacegroup name	C 2 2 2
Unit cell lengths	34.869, 66.474, 77.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.690 - 2.000
R-factor	0.219
Rwork	0.213
R-free	0.27500
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.514
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.689	30.540	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.052	0.034	0.757
Total number of observations		1899	8036
Number of reflections	6383
<I/σ(I)>	9.135	15.4	1
Completeness [%]	100.0	99.3	99.9
Redundancy	9.2	8	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	291	0.4 M NH4(SO4)2, 1 M LiSO4, 0.1 M Na Citrate, pH 6.2, vapor diffusion, hanging drop, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.2	291	0.4 M NH4(SO4)2, 1 M LiSO4, 0.1 M Na Citrate, pH 6.2, vapor diffusion, hanging drop, temperature 291K