3EAT

Crystal structure of the PvcB (PA2255) protein from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	113
Detector technology	CCD
Collection date	2006-05-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	125.350, 125.350, 107.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.189
Rwork	0.187
R-free	0.22500
RMSD bond length	0.010
RMSD bond angle	1.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.083	0.484
Number of reflections	17734
<I/σ(I)>	19.1	2.6
Completeness [%]	99.9	99.8
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	2-5% PEG 20000, 75-150 mM NaCitrate, 100 mM BTP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K