3E9D
Structure of full-length TIGAR from Danio rerio
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-14 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.661, 115.541, 61.311 |
| Unit cell angles | 90.00, 104.66, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.140 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.076 | 0.033 | 0.425 |
| Number of reflections | 57061 | ||
| Completeness [%] | 96.1 | 98.1 | 79.3 |
| Redundancy | 3 | 3.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 1 M sodium phosphate, pH 6.5, vapor diffusion, temperature 293K |
