3E7N
Crystal structure of d-ribose high-affinity transport system from salmonella typhimurium lt2
Replaces: 3DSAExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 293.042, 92.726, 93.601 |
Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.450 |
R-factor | 0.19279 |
Rwork | 0.191 |
R-free | 0.23255 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.398 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.120 | 0.750 |
Number of reflections | 88248 | |
<I/σ(I)> | 13.4 | 2.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M Bis-tris 20% PEG MME 5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |