3E7K
Crystal Structure of an antiparallel coiled-coil tetramerization domain from TRPM7 channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.258, 35.674, 100.322 |
| Unit cell angles | 90.00, 124.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.010 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.783 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.121 | 0.359 |
| Number of reflections | 28234 | |
| Completeness [%] | 97.7 | 85.4 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1 M MgCl2, 0.1 M Tris, 5-10% Isopropanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
